IPTG References

1. Assembly and analysis of eukaryotic translation initiation complexes
   AV Pisarev, A Unbehaun, CUT Hellen… - Methods in enzymology, 2007 - Elsevier ...
   Isopropyl-β-d-thiogalactopyranoside (IPTG) was from Bioworld (Dublin, OH), GMP-PNP and Pefabloc were from Roche (Indianapolis, IN), protease inhibitor cocktail set III was from Calbiochem (La Jolla, CA), puromycin was from EMD Biosciences (San Diego, CA), and ...
   
2. In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entry
   VG Kolupaeva, S Breyne, TV Pestova… - Methods in enzymology, 2007 - Elsevier ... Louis, MO).
   The protease inhibitor cocktail set III and puromycin are from Calbiochem (La Jolla, CA), GMP-PNP and Pefabloc are from Roche (Indianapolis, IN), and isopropyl-β-d-thiogalactopyranoside (IPTG) is from Bioworld (Dublin, OH). ...
   
3. Recent advances in the study of rTS proteins. rTS expression during growth and in response to thymidylate synthase inhibitors in human tumor cells
   BJ Dolnick, K Lu, MB Yin… - Advances in Enzyme Regulation, 1997 - Elsevier ... MA).
   Amiigase (thermally stable DNA ligase, EC 6.5.1.1) and gelase (agarase, EC 3.2.1.81) were obtained from Epicentre (Madison, WI). IsopropylthioDgalactoside (IPTG) was from BioWorld (Dublin, OH). Oli godeoxyribonucleotides ...
   
4. Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in …
   CE Noriega, V Sharma… - Journal of bacteriology, 2007 - Am Soc Microbiol
   ... The antibiotics (BioWorld, Dublin, OH) used for E. coli were as follows: ampicillin (100 µg/ml), gentamicin (15 µg/ml), and ... Intermediate levels of nitrate reductase activities can even be obtained using IPTG (isopropyl-ß-D-thiogalactopyranoside) (data not shown). ...
   
5. From no expression to high-level soluble expression in Escherichia coli by screening a library of the target proteins with randomized N-termini
   KH Kim, JK Yang, GS Waldo… - METHODS IN …, 2008 - Springer
   ... 5. 1% (w/v) Agarose gel (Bioworld, Dublin, OH). 6. 12.5% (w/v) SDS-PAGE gel. ... Aliquot in 1 ml fractions and store at −20°C. 6. Isopropyl-β-D-thio-galactopyranoside (IPTG) stock solution (1.0 M): 4.8 g IPTG in 20 ml of sterile water. ..
   
6. Human {alpha}-Defensins Inhibit Hemolysis Mediated by Cholesterol-Dependent Cytolysins
   RI Lehrer, G Jung, P Ruchala, W Wang… - Infection and …, 2009 - Am Soc Microbiol
   ... Cytotoxins. Recombinant LLO was purchased from bio-WORLD (Dublin, OH). ... ALO expression was induced by adding 0.5 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) when the cultures reached an optical density at 600 nm of 1.0 to 1.2. ...
   
7. Transcription regulation is demonstrated for five key enzymes in Giardia intestinalis cyst wall polysaccharide biosynthesis
   AB Lopez, K Sener, EL Jarroll… - Molecular and biochemical …, 2003 - Elsevier
   ... expressed by diluting cultures, grown for 16 h in LB medium, 1:1 with fresh medium having a final IPTG concentration of 1 ... directed against GNA, AGM, and UAP were generated by Rockland (Gilbertsville, PA); the UAE antiserum was prepared by Bio-World (Dublin, OH). ...
   
8. Interaction of Proteus mirabilis urease apoenzyme and accessory proteins identified with yeast two-hybrid technology
   SR Heimer… - Journal of Bacteriology, 2001 - Am Soc Microbiol
   ... at 37°C with aeration, induced in mid-exponential phase with 0.3 mM isopropyl- -D-thiogalactopyranoside (IPTG), and incubated for ... Purified UreC and UreD proteins embedded in SDS-polyacrylamide were supplied to BioWorld Laboratories (Dublin, Ohio) for the ...
   
9. Enhanced protein thermostability and temperature resistance
   FT Robb… - US Patent App. 20,040/ …, 2003 -
   ... [0084] The Pfu-sHSP expression was induced by 1 mM isopropyl-1-thio-β-D-galacgtopyranoside (IPTG) for 3 hours. ... antibody preparations against the Pfu-sHSP were obtained by immunization of a rabbit with the purified, recombinant Pfu-sHSP (Bio-world, Dublin, Ohio). ...
   
10. BfpU, a soluble protein essential for type IV pilus biogenesis in enteropathogenic Escherichia coli
    W Schreiber, KD Stone, MA Strong… - …, 2002 - Soc General Microbiol
    ... Ascites was prepared commercially (BioWorld, Dublin, OH) and purified using ... Bacteria were grown under BFP-inducing conditions and centrifuged (4000 g, 15 min at 4 °C). IPTG (1 mM) was added to the cultures for the last hour of incubation to induce ß-galactosidase ...
    
11. Identification of a phosphate regulatory site and a low affinity binding site for glucose 6-phosphate in the N-terminal half of human brain hexokinase
    TY Fang, O Alechina, AE Aleshin, HJ Fromm… - Journal of Biological …, 1998 - ASBMB
    ... Isopropyl-1-thio-β-d-galactopyranoside (IPTG) was from BioWorld. ... are: Glu-6-P, glucose 6-phosphate; 1,5-AnG-6-P, 1,5-anhydroglucitol 6-phosphate; Glu-1,6-P 2 , glucose 1,6-bisphosphate; IPTG, isopropyl-1 ...
    
12. Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene
    R Alvarez, BS Seal - Virology journal, 2005 -
    ... concentration of the supernatant fraction from infected cells was measured for protein concentration by Bradford's reagent (Bioworld, Dublin, OH) at 595 nm. ... Protein was induced with IPTG at 24 hours post-transfected and total proteins were ...
    
13. Nonaggregating mutant of recombinant human hexokinase I exhibits wild-type kinetics and rod-like conformations in solution
    AE Aleshin, M Malfois, X Liu, CS Kim… - Biochemistry, 1999 - ACS Publications
    ... Isopropyl-1-thio-β-d-galactopyranoside (IPTG) was from BioWorld. ... stirring of 200 rpm and a filtered air flow of 6 L/min) at 37 °C to early log phase (A 600 = 0.4), after which isopropyl-d- thiogalactopyranoside (IPTG) was added to ... 1. Lowry, OH, and Passonneau, JV (1964) J. Biol. ...
    
14. Heterologous expression of functional Ptr ToxA
    RP Tuori, TJ Wolpert… - Molecular plant-microbe …, 2000 - Am Phytopath Society
    ... used to inoculate 25 ml of fresh LBA and incubated with shaking at 37°C for 1 h. Expression of fusion proteins was induced by addition of isopropyl-beta-D- thiogalactopyranoside (IPTG), to a ... Antiserum against refolded, purified NC-FP was raised at Bioworld (Dublin, OH). ...